QTL analysis and development of HRM markers associated with fruit shape in interspecific pears (Pyrus pyrifolia × P. bretschneideri)
نویسندگان
چکیده
Fruit shape is one of the important quantitative traits in pear (Pyrus spp.) breeding program, thus genetic study related to fruit could be beneficial breeding. Quantitative trait loci (QTL) analysis was carried out using ‘Whangkeumbae’ (P. pyrifolia, round) × ‘Yali’ bretschneideri, pyriform) and high-resolution melting (HRM) markers were developed. The linkage map constructed single nucleotide polymorphisms (SNPs) derived from Axiom Pear 70 K Genotyping Array simple sequence repeats. integrated showed ~ 90% genome coverage, a total distance 998.2 cM, marker density 1.6 cM. F1 progenies normal distribution length (L), diameter (D), L/D ratio. Three QTLs located group (LG) 6, 7, 12 identified with LOD thresholds 2.8–3.0. Six HRM developed array-SNPs anchored predicted 28.6–65.3% accuracy. Notably, accuracy increased by an combination consisting CBp06sn02, CBp07sn01, CBp12sn03. These results provide better understanding mechanism development reducing period.
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ژورنال
عنوان ژورنال: Euphytica
سال: 2022
ISSN: ['1573-5060', '0014-2336']
DOI: https://doi.org/10.1007/s10681-022-03079-9